TABLE 2 .
Primers and probes used for qRT-PCR validation of NGS dataa
| Transcription gene | Exon(s) | Sequence | 5′ modification | 3′ modification |
|---|---|---|---|---|
| TNF | 2, 3, and 4 | CCAGGCAGTCAGATCATCTTCTCTCTCAGCTCCACGCCATTGCCCGAGTGACAAGCCTGTAGCCCAT | 6-FAM | BHQ-1 |
| IFN-B1 | 1 | CTTTCCATGAGCTACAACTTGCTTATCTCCTCAGGGATGTCAAAGTTCCAATTTTCAGTGTCAGAAGCTCCTGTGGC | 6-FAM | BHQ-1 |
| IFN-B1 | 1 | CAGCAATTTTCAGTGTCAGAAGCTCATCCTGTCCTTGAGGCAGTATTAAGCCTCCCATTCAATTGCCA | 6-FAM | BHQ-1 |
| ADPGK | 4 and 5 | CTTCTTGATGACAATGTCTTTGTTCTTTAACTGGCCCCACTCCTCATCCACTTCCTGCAATGACTCTGGT | CFG540 | BHQ-1 |
a
6-FAM, 6-carboxyfluorescein; BHQ-1, black hole quencher 1.