FIG 4 .

Detection of TBP2 conjugates modified by SAMPs. TBP2 was enriched from wild-type (wt) and SPS deletion strains by Strep-Tactin (anti-StrepII antibody) (A and B) and tandem affinity purification (C), which included a subsequent immunoprecipitation by anti-Flag antibody. TBP2-StrepII was ectopically expressed with Flag-SAMP2 in the wt and SPS mutant strains and compared to an empty-vector control. Equivalent amounts of cell lysates, as determined by BCA assay, were applied to the Strep-Tactin resin. TBP2, TBP2 conjugates (including the Ubl-modified form, SAMP2-TBP2), and an anti-StrepII antibody signal likely to be a TBP2 dimer (*) are indicated. See Materials and Methods for details.