TABLE 1 .
Relative growth rates of A. baumannii BM4587 and derivatives overexpressing each pump or with each pump deleted
|
A. baumannii strain |
Mutationa | Pump overexpression rate (fold)a,b |
Relative growth rate (min−1)c |
Reference |
|---|---|---|---|---|
| BM4587 | Wild-type | 1 | 1.00 | 33 |
| BM4688 | AdeSR152K | 38 for adeB | 0.96 ± 0.05 | 9 |
| BM4689 | AdeRA91V | 222 for adeB | 0.91* ± 0.04 | 9 |
| BM4717 | ΔadeB | NA | 1.00 ± 0.03 | 9 |
| BM4690 | AdeLN334H | 402 for adeG | 0.82* ± 0.02 | 9 |
| BM4691 | AdeLQ332stop | 636 for adeG | 0.81* ± 0.03 | 9 |
| BM4718 | ΔadeG | NA | 1.00 ± 0.03 | 9 |
| BM4666 | adeNΔC584 | 6 for adeJ | 0.94* ± 0.03 | 33 |
| BM4719 | ΔadeJ | NA | 0.98 ± 0.01 | 9 |
Data taken from reference 9.
The pump overexpression rate values are shown as the fold or number of times the strain overexpressed the indicated gene. For instance, strain BM4688 overexpressed adeB 38-fold compared to the wild-type or parental BM4587 strain. NA, not applicable.
The growth rates of derivatives overexpressing each RND efflux system or with each efflux system deleted were determined at the beginning of the exponential phase. The relative growth rate represents the ratio of growth of an isogenic derivative to that of the parental (wild-type) strain taken as 1.00. Every isolate was tested in duplicate in four to six independent experiments, and the results are presented as means ± SD. The mean values that are significantly different (P < 0.05 by the Student t test) from the value for the parental strain are indicated by an asterisk.