Figure 3.

Intracellular Alexa Fluor 594 did not alter the rectification index of EPSCs mediated by GluR2-containing AMPA receptors. Mice were exposed to fox urine for 5 min, a treatment that increased GluA2-containing AMPARs and therefore the initial rectification index of EPSCs. Cerebellar slices were prepared 3 h later, and sEPSCs were recorded with a pipette solution that included Alexa 594. A, Example trace of average synaptic currents (top, +40 mV; bottom, −60 mV; lines: black, 0–5 min; orange, 15–20 min). B–D, Average and individual sEPSC rectification index (B) and amplitude (C) recorded at −60 mV and +40 mV (D). Neither the RI nor the amplitude of EPSCs changed after 15–20 min of recording (5 cells, 5 animals). Bar graphs represent the average, and open circles represent individual values.