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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Nutr Biochem. 2015 Sep 28;33:54–62. doi: 10.1016/j.jnutbio.2015.09.014

Fig. 5.

Fig. 5

UA decreases Nrf2 promoter DNA methylation in JB6 P+ cells. The cells were treated with each concentration of UA for 3 days, and then the genomic DNA was isolated for bisulfite conversion. The methylation status of the first 15 CpG sites, the region between -1226 and -863 relative to the translational start site, within the promoter of Nrf2, was analyzed. Positive control cells were treated with 5-aza (250 nM) for 48 h and TSA (50 nM) for 24 h. Ten individual clones were analyzed. The filled and open dots indicate methylated and unmethylated CpG. The data are expressed as a percentage of the total number of methylated cytosines vs. total 15 CpGs of three independent experiments.

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