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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Nat Protoc. 2016 Mar 31;11(5):853–871. doi: 10.1038/nprot.2016.043

Figure 3. Flow chart of bioinformatic pipeline for translocation junction identification.

Figure 3

Multiple HTGTS libraries with different barcodes can be sequenced in the same Miseq flow cell. De-multiplexing separates sequencing reads for each library, followed by sequence read processing which takes into account bait, prey, and adapter alignments to optimally define the sequence read. Uniquely mapped bait-prey junctions are retained as filtered junctions while identical junctions are separated.