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. 2016 Jun 7;6:27438. doi: 10.1038/srep27438

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Copyright © 2016, Macmillan Publishers Limited

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(A) Antioxidant activity of BxPrx transformants was investigated using a discs assay. (B) Difference of susceptibility to H₂O₂ between E. coli with pET-28a-BxPrx and blank pET-28a transformed were compared by the killing zone diameter. (C) DNA protection assay. Free oxidant radical attack was generated due to the presence of Fe³⁺ and DTT. PUC19 plasmid was incubated for 0.5, 1, 2, and 4 h respectively. After attacked by the radical, supercoiled plasmid (SC) was degraded to slower-moving nicking (N), and then slowest linear DNA (L).