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. 2016 Jun 7;6:27112. doi: 10.1038/srep27112

Figure 2. CtsD inhibition reduces tubular cell injury and apoptosis in FA nephrotoxic induced AKI.

Figure 2

Representative PAS pictures, damaged cells pointed with a black star *. (A) Percentage of tubular cell injury in cortex (B) as assessed by tubular dilatation, epithelial flattening and loss of brush border in FA vehicle or Pepstatin A treated kidneys. Average number of NIMP+ cells per field in FA vehicle or Pepstatin A treated kidneys. (C) Cleaved caspase-3 and GAPDH WB (D) of control vehicle and FA vehicle or Pepstatin A treated kidneys. Percentage of cortical TUNEL positive cells versus total cells and representative TUNEL only or DAPI merged pictures (E) in FA vehicle or Pepstatin A treated kidneys. White arrows point to TUNEL+ cells. Animals were treated with vehicle or Pepstatin A (20 mg/Kg) 45 minutes before and 24 hours post-FA. N = 8, t-test, *P ≤ 0.05 or **P ≤ 0.01.