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. 2016 Jun 7;6:27112. doi: 10.1038/srep27112

Figure 5. Pepstatin A reduces apoptosis with no alteration on neutrophil infiltration in IRI induced AKI.

Figure 5

Average number of NIMP+ cells per field with representative pictures in the CMJ of IRI vehicle or Pepstatin A treated kidneys. (A) CXCL-1 and CXCL-2 mRNA expression from sham and IRI vehicle or Pepstatin A treated kidneys. (B,C) Cleaved caspase-3 and GAPDH WB (D) of sham, control or IRI vehicle or Pepstatin A treated kidneys. Percentage of CMJ TUNEL positive cells versus total cells and representative TUNEL only or DAPI merged pictures (E) in IRI vehicle or Pepstatin A treated kidneys. Ischemia was performed for 25 minutes and kidneys were reperfused for 24 hours. Animals were treated with vehicle or Pepstatin A 10 mg/Kg 1 hour before surgery and 4 hours post-surgery. N = 7, 1 way ANOVA or t-test,*P ≤ 0.05 or **P ≤ 0.01.