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. 2016 Jun 7;6:27112. doi: 10.1038/srep27112

Figure 7. Pepstatin A pre-treatment reduces interstitial fibrosis development from IRI induced AKI.

Figure 7

CtsD fluorometric activity in kidney lysates assessed by the cleavage of a specific fluorescently labelled substrate. (A) Cleaved caspase-3 and GAPDH WB (B) in sham and IRI vehicle or Pepstatin A treated animals. Morphometric analysis of SR+ area/field (C) or α-SMA+ area/field (D) of kidney cortex from sham and IRI vehicle or Pepstatin A treated kidneys. Col1A1 (E) and Col3A1 (F) mRNA expression from sham and IRI vehicle or Pepstatin A treated kidneys. Ischemia was performed for 35 minutes and kidneys were reperfused for 28 days. Animals were treated with vehicle or Pepstatin A 20 mg/Kg 1 hour before surgery and from day 2 post-surgery three times a week up to 28 days. N = 6, 1 way ANOVA or t-test,*P ≤ 0.05 or **P ≤ 0.01.