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. 2016 May 3;15(6):1955–1962. doi: 10.1021/acs.jproteome.6b00127

Figure 3.

Figure 3

Fluorescence imaging and MALDI MSI of B. subtilis biofilms of NCIB3610 and its mutants integrated with the PyqxM-CFP reporter. CFP images were acquired using a fluorescence stereoscope before MALDI analysis. Each individual column represents (left to right): m/z 1030 (surfactin-C13, [M + Na]+); m/z 1044 (surfactin-C14, [M + Na]+); m/z 1058 (surfactin-C15, [M + Na]+); m/z 1485 (plipastatin-C16-Ala, [M + Na]+); m/z 1499 (plipastatin-C17-Ala, [M + Na]+); m/z 1513 (plipastatin-C16-Val, [M + Na]+); m/z 1527 (plipastatin-C17-Val, [M + Na]+); m/z 2782 (SKF, [M + H]+), m/z 3422 (subtilosin, [M + Na]+), m/z 4334 (SDP, [M + Na]+), and an overlay of ion images (green: SKF; bright pink: SDP). The ion intensity is reflected by the intensity of colors. Each column of ions is displayed using the same intensity scale, optimized per each metabolite and normalized to the TIC. Scale bar = 2 mm for fluorescence images and 5 mm for optical and ion images.