Table 4.
The top 40 differentially expressed genes making the greatest contribution to the inter-strain differences in hypothalamic function of ISIAH and WAG rats
| Gene symbol | Acc.# | Correlation | log2 (fold_change) ISIAH/WAG | Definition | Function and/or process |
|---|---|---|---|---|---|
| Retsat | NM_145084 | 0.998 | 1.82 | retinol saturase (all trans retinol 13,14 reductase) | oxidoreductase activity [64] |
| Sc5d | NM_053642 | 0.997 | 1.21 | sterol-C5-desaturase | cholesterol biosynthetic process via lathosterol [65] fatty acid metabolic process; lathosterol oxidase activity [66] |
| RGD1359290 | NM_001047898 | 0.996 | was detected in ISIAH but not in WAG | Ribosomal_L22 domain containing protein RGD1359290 | structural constituent of ribosome |
| Abcg2 a | NM_181381 | 0.994 | 1.83 | ATP-binding cassette, subfamily G (WHITE), member 2 | transmembrane transport [67] |
| Ephx2 a,b | NM_022936 | 0.993 | 4.40 | epoxide hydrolase 2, cytoplasmic | positive regulation of blood pressure [24] |
| Fam111a | NM_001109163 | 0.993 | 5.12 | family with sequence similarity 111, member A | DNA replication [68] |
| RGD1563482 a | NM_001109065 | 0.990 | 0.79 | similar to hypothetical protein FLJ38663 | aminoacyl-tRNA hydrolase activity |
| Stra6 | NM_001029924 | 0.990 | 0.70 | stimulated by retinoic acid 6 | transports retinol across the cell membrane [18] |
| Aox2 | NM_001008522 | 0.985 | 1.47 | aldehyde oxidase 2 | oxidoreductase activity |
| Fhit | NM_021774 | 0.983 | 1.46 | fragile histidine triad | nucleotide metabolic process [69] |
| Tekt4 | NM_001013965 | 0.981 | 1.00 | tektin 4 | protein binding [70] |
| LOC689064 | NM_001111269 | 0.980 | 3.23 | beta-globin | oxygen transporter activity |
| Grhl3 | NM_001106690 | 0.976 | 1.37 | grainyhead-like 3 (Drosophila) | may stimulate migration of endothelial cells [71] |
| Acad9 | NM_181768 | 0.975 | 0.59 | acyl-CoA dehydrogenase family, member 9 | oxidoreductase activity catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA |
| Tgfb2 a | NM_031131 | 0.975 | 0.85 | transforming growth factor, beta 2 | promotes the survival of dopaminergic neurons [37] |
| Spg7 | NM_181388 | 0.975 | 0.58 | spastic paraplegia 7 homolog (human) | metalloendopeptidase activity [72] nervous system development [73] |
| Idnk | NM_001037362 | 0.974 | 0.91 | idnK, gluconokinase homolog (E, coli) | gluconokinase activity |
| Lyz2 | NM_012771 | −0.968 | −0.68 | lysozyme 2 | lysozyme activity |
| Mal a | NM_012798 | −0.971 | −0.72 | mal, T-cell differentiation protein | myelination [74]; structural constituent of myelin sheath [75] |
| Lcat | NM_017024 | −0.972 | −0.77 | lecithin cholesterol acyltransferase | phospholipase A2 activity [76] cholesterol metabolic process [77] |
| PCOLCE2 | NM_001127640 | −0.972 | −2.12 | procollagen C-endopeptidase enhancer 2 | collagen binding [78] |
| RT1-S3 | NM_001008886 | −0.974 | −2.03 | RT1 class Ib, locus S3 | antigen processing and presentation of peptide antigen via MHC class I |
| Cst3 a,b | NM_012837 | −0.976 | −0.59 | cystatin C | prevents oxidative stress-induced death [44] |
| Cyp2j10 | NM_001134980 | −0.977 | −0.94 | cytochrome P450, family 2, subfamily j, polypeptide 10 | EETs biosynthesis [79] |
| Gstm4 | NM_001024304 | −0.978 | −1.75 | glutathione S-transferase mu 4 | glutathione binding glutathione metabolic process |
| Grm2 | NM_001105711 | −0.979 | −1.81 | glutamate receptor, metabotropic 2 | regulation of synaptic transmission, glutamatergic [80] |
| Mx2 | NM_134350 | −0.979 | −1.29 | myxovirus (influenza virus) resistance 2 | innate immune response [81] |
| Zfp385d | NM_001013992 | −0.980 | −0.74 | zinc finger protein 385D | zinc ion binding |
| Tnnt1 | NM_001277260 | −0.981 | −4.37 | troponin T type 1 (skeletal, slow) | regulation of muscle contraction [82] |
| Cpz | NM_031766 | −0.982 | −0.89 | carboxypeptidase Z | zinc ion binding metallocarboxypeptidase activity extracellular matrix homeostasis |
| Pyroxd2 | NM_001004261 | −0.983 | −2.86 | pyridine nucleotide-disulphide oxidoreductase domain 2 | oxidoreductase activity |
| Rbp4 | NM_013162 | −0.985 | −1.49 | retinol binding protein 4, plasma | retinol transporter activity [83] |
| Abca8a | NM_001281824 | −0.987 | −0.65 | ATP-binding cassette, subfamily A (ABC1), member 8a | transporter activity lipid transport transmembrane transport |
| Mpeg1 | NM_022617 | −0.989 | −2.31 | macrophage expressed 1 | |
| Grifin | NM_057187 | −0.989 | −4.44 | galectin-related inter-fiber protein | carbohydrate binding |
| Ltbp2 b | NM_021586 | −0.989 | −2.88 | latent transforming growth factor beta binding protein 2 | decreases fibroblast adhesion to fibronectin [84] negatively regulates coalescence of oxytalan fibers induced by stretching stress [85] |
| Snx14 | NM_001108174 | −0.990 | −0.85 | sorting nexin 14 | phosphatidylinositol-3,5-bisphosphate binding [86] |
| Pnpla1 | NM_001191841 | −0.993 | −2.59 | patatin-like phospholipase domain containing 1 | lipid catabolic process |
| RT1-A2 | NM_001008829 | −0.993 | −1.98 | RT1 class Ia, locus A2 | antigen processing and presentation of peptide antigen via MHC class I |
| RGD1309362 | NM_001024884 | −0.995 | −2.46 | similar to interferon-inducible GTPase |
Genes associated with: acentral nervous system diseases; b-hypertension; ISIAH and WAG – rat strains used in the study