. 2016 Jan 27;16(Suppl 1):9. doi: 10.1186/s12870-015-0691-2

Table 1.

PCR primers details

Primers	Primer sequence (5'-3')	Primer design	Annealing temp. °C	Amplified region	Allelic variant	PCR product size (bp)
VRN1AF	gaaaggaaaaattctgctcg	Yan et al. [19]	60	VRN-A1 promoter	vrn-A1	713
VRN1-INT1R	gcaggaaatcgaaatcgaag	Yan et al. [19]	60	VRN-A1 promoter	Vrn-A1a.1	853, 944
					Vrn-A1a.2	853, 924, 944
					Vrn-A1a.3	765,853
					vrn-A ^m 1	705
					Vrn-A1b	691
					Vrn-A1d	685
					Vrn-A ^m 1g	681
					Vrn-A ^m 1a	671
					Vrn-A1e	659
					Vrn-A1f	658
					vrn-A ^m 1b	656
Vrn-A1-intr_F	ccgtcgaaaggatcgctactg	This study	60	VRN-A1 intron-1	vrn-A1	541
Vrn-A1-intr_R1	cttgtccccgtgagctacttac	This study	60	VRN-A1 intron-1
Ex1/C/F	gttctccaccgagtcatggt	Fu et al. [20]	58	VRN-A1 intron-1	Vrn-A1c (Langdon)	522
Intr1/A/R3	aagtaagacaacacgaatgtgaga	Fu et al. [20]	58	VRN-A1 intron-1	Vrn-A1c (IL369)	2188
Pr1	tacccctgctaccagtgcct	Shcherban et al. [22]	60	VRN-B1 promoter	VRN-B1.f	968
Pr2	ggccaaccctacaccccaag		60	VRN-B1 promoter	VRN-B1.s	958
					VRN-B1.m	965
Pr1	tacccctgctaccagtgcct	Shcherban et al. [22]	60	VRN-B1 promoter	Vrn-B1(ins)	1256
PrB/Ins/r2	tgcggtacgggttatccag	This study	60	VRN-B1 promoter
Ex1/C/F	gttctccaccgagtcatggt	Fu et al. [20]	61	VRN-B1 intron-1	Vrn-B1a	1091
Intr1/B/R3	ctcatgccaaaaattgaagatga		61	VRN-B1 intron-1	Vrn-B1b	1055
					Vrn-B1c	705
Ex1/C/F	gttctccaccgagtcatggt	Fu et al. [20]	61	VRN-B1 intron-1	vrn-B1	1531
Intr1/B/R4	caaatgaaaaggaatgagagca	Fu et al. [20]	61	VRN-B1 intron-1
FT-B-INS-F	cataatgccaagccggtgagtac	Yan et al. [3]	63	VRN-B3 promoter	Vrn-B3a	1765
VRN4-B-NOINS-R	ctatccctaccggccattag	Yan et al. [3]	63	VRN-B3 promoter
VRN4-B-NOINS-F2	gctgtgtgatcttgctctcc	Yan et al. [3]	63	VRN-B3 promoter	vrn-B3	691
VRN4-B-NOINS-R	ctatccctaccggccattag	Yan et al. [3]	63	VRN-B3 promoter	vrn-B3b	1581