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. 2014 May 28;1(2):26–36. doi: 10.1002/reg2.11

Figure 3.

Figure 3

RT‐PCR analysis of the induced bump. Gene expression pattern was investigated by RT‐PCR. Samples were prepared from the induced bumps (2 weeks after the surgery), regular blastemas (MB; 2 weeks after amputation), and intact skin. Msx1b, Fgf10, Fgf8, and Prrx1 were positive in both the bump and the regular blastema, while they were negative in intact skin. Hoxa13 could not be detected in the bump but could be detected in the regular blastema. Bmp genes were also investigated by RT‐PCR. Bmp2, 4, and 7 were positive in both the bump and the regular blastema. Type II collagen and Sox9 were unexpectedly detectable in the bump, but the regular blastema showed much higher expression level. Ef1α was an internal control.