Figure 5.

Cartilaginous spike induction by deep wounding. (A) Time course of the induced bump and spike induction. Skin wounding plus nerve deviation result in bump formation. In this case, a small bump can be seen within 12 days, but it starts regressing by day 19. On the other hand, when a deep wound is created enough to damage a stylopod bone, a cartilaginous spike can be observed. In this case, a relatively smaller bump can be observed at day 12. The induced bump kept growing up to spike formation. (B, C) Induced cartilaginous spike at day 45. (D) Whole‐mount Alcian blue staining. Scale bars in (A), (B), (C), and (D) are 2 mm.