Table 1.
Survival rate of embryos injected with various concentrations of TALEN mRNA or left uninjected. For each condition, up to 10 individual embryos were injected initially. Embryos without successful fertilization were excluded from the experiment. At two days post‐injection, two embryos from each group were harvested for DNA isolation and the subsequent survival rate was determined according to the adjusted total animal numbers. Survival was determined by visual inspection
| Survival rate | |||||||
|---|---|---|---|---|---|---|---|
| Total mRNA injected (pg) | 1 day | 2 daysa | 4 days | 6 days | 15 days | 18 weeks | |
| WT | N.A. | 86% (6/7) | 80% (4/5) | 80% (4/5) | 80% (4/5) | 80% (4/5) | 80% (4/5) |
| TALEN1 | 300 | 88% (7/8) | 83% (5/6) | 0% (0/6) | 0% (0/6) | 0% (0/6) | 0% (0/6) |
| 200 | 90% (9/10) | 88% (7/8) | 13% (1/8) | 13% (1/8) | 13% (1/8) | 13% (1/8) | |
| 100 | 100% (9/9) | 100% (7/7) | 43% (3/7) | 29% (2/7) | 14% (1/7) | 0% (0/7) | |
| 50 | 100% (8/8) | 100% (6/6) | 83% (5/6) | 83% (5/6) | 67% (4/6) | 33% (2/6) | |
| TALEN2 | 300 | 100% (8/8) | 33% (2/6) | 0% (0/6) | 0% (0/6) | 0% (0/6) | 0% (0/6) |
| 200 | 88% (7/8) | 67% (4/6) | 0% (0/6) | 0% (0/6) | 0% (0/6) | 0% (0/6) | |
| 100 | 100% (8/8) | 100% (6/6) | 50% (3/6) | 50% (3/6) | 0% (0/6) | 0% (0/6) | |
| 50 | 100% (8/8) | 83% (5/6) | 67% (4/6) | 50% (3/6) | 50% (3/6) | 50% (3/6) | |
a. Two embryos from each group were harvested for DNA isolation at 2 days post‐injection. Remaining animals (N‐2) were subsequently used to determine survival rate. N = initial number of embryos injected.