Figure 2. Impaired hematopoiesis and loss of progenitors and HSCs in FL and BM of hnRNP L deleted mice.

(a) Absolute cell numbers of granulo-myeloid-erythroid progenitors GMP (defined as Lin⁻c-Kit⁺Sca-1⁻CD34⁺CD16/32⁺), CMP (defined as Lin⁻c-Kit⁺Sca-1⁻CD34⁺CD16/32⁻) and MEP (defined as Lin⁻c-Kit⁺Sca-1⁻CD34⁻CD16/32⁻). Shown are data from control mice (hnRNP L^fl/fl) or animals where hnRNP L was deleted in FL (VavCre⁺hnRNP L^fl/fl) or in adult BM (MxCre⁺hnRNP L^fl/fl) (n = 3 or more). (b) Absolute cell numbers of common lymphoid progenitor (CLP) in both FL (n = 5) and BM (n = 6) of control or hnRNP L deficient mice. (c) Absolute cell numbers of HSCs (defined as Lin⁻Sca1⁺c-Kit⁺ (LSK) Flt3⁻CD150⁺CD48⁻), MPP1 (defined as LSK Flt3⁻CD150⁺CD48⁺) and MPP2 (defined as LSK Flt3⁻CD150⁻CD48⁻) from FL control mice (hnRNP L^fl/fl) and VavCre⁺hnRNP L^fl/fl. (d) Absolute cell numbers of HSCs, MPP1 and MPP2 from BM of control mice (hnRNP L^fl/fl) and MxCre⁺hnRNP L^fl/fl (n = 3 or more). All error bars are means ± SEM (*p < 0.01, **p < 0.001, ***p < 0.0001).