Table 2. K ds, Hill coefficients (shown in parentheses) and stoichiometries of 2, AU-azide and 2AU-2 binding to RNA repeats.
| RNA repeats | r(AUUCU)11 |
r(CUG)12* |
r(CGG)12* |
r(CCUG)12* |
||||
|---|---|---|---|---|---|---|---|---|
| Compound | Kd, nM | Stoichiometry | Kd, nM | Stoichiometry | Kd, nM | Stoichiometry | Kd, nM | Stoichiometry |
| 2 | 300±47 (1.7±0.2) | 11±0.5 | ∼4,000 | ∼7 | ∼2,000 | ∼6 | ∼4,000 | ∼11 |
| AU-azide | 375±34 (1.3±0.1) | 13±0.9 | ∼5,000 | ∼11 | ∼3,000 | ∼9 | ∼5,000 | ∼16 |
| 2AU-2 | 185±1.0 (1.4±0.4) | 7±0.1 | ∼4,000 | ∼9 | ∼4,000 | ∼6 | ∼4,000 | ∼6 |
*The stoichiometry from unsaturated binding curves was estimated from data fit by equation (1). The Kd was calculated by using the estimated stoichiometry.