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. 2016 Jun 7;6:27463. doi: 10.1038/srep27463

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Copyright © 2016, Macmillan Publishers Limited

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(a) Percentage remaining biofilm cfus in wild type (CA-IF100) and triple sod mutant (ΔΔΔsod4sod5sod6) after treatment with 30 μM miconazole. A two-tailed paired Students t-test was performed to assign significant differences between the two strains (*p < 0.05). (b) Number of cfus per C. albicans SC5314 biofilm and (c) concomitant accumulation of superoxide radicals in the presence and absence of miconazole and/or DDC. Black bars represent untreated biofilms or biofilms treated with 10 mM DDC alone while grey bars represent biofilms additionally treated with 125 μM miconazole. Data presented are the mean and SEM of four (n = 4) independent experiments (each consisting of three replicates). Two-way ANOVA statistical analysis with Tukey’s correction for multiple comparisons was performed to assign significant differences between treatments (****p < 0.0001).