Table 1. Selection of inhibitors and inducers of the miconazole-affected pathways and combinatory action with miconazole against C. albicans biofilms determined by FICI calculation.

Process or protein	Effect in miconazole RNAseq data	Compound	BEC50	Activity in combination with miconazolea
		Inhibitors
Sterol biosynthetic process	Induced	Simvastatin	1.4 mM	S
Secreted aspartic-type proteinases	Induced	Saquinavir mesylate	>500 μM	I
Lipases	Induced	Orlistat	>1 mM	I
Glyoxylate cycle	Induced	Itaconate	>2 mM	I
ETC	Repressed	Antimycin A	570 μM	S
		CCCP	73 μM	S
		Sodium azide	8.6 mM	S
Sphingolipid biosynthesis	Induced	Myriocin	>100 μM	I
Alcohol dehydrogenase	Induced	Disulfiram	>500 μM	I
Branched-chain amino acid (including leucine) biosynthesis	Induced	Chlorosulfuron	>1 mM	I
Fe-S bonds (as in Leu1 and many mitochondrial respiratory enzymes)	Induced	Pyrithione zinc	275 μM	S
Tetrahydrofolate interconversion	Induced	Trimethoprim	>500 μM	I
		Inducers
Nicotinic acid transporter	Induced	Nicotinic acid	>1 mM	I
Arginine biosynthesis	Repressed	Arginine	>10 mM	I
Leucine biosynthesis	Induced	Leucine	>2 mM	I
Citric acid cycle	Repressed	Sodium dichloroacetate	>1 mM	I
N-acetylglucosamine transporter	Repressed	N-acetyl-D-glucosamine	>1 mM	I

^aS = Synergy (FICI < 0.5), I = Indifference (0.5 < FICI < 4), FICI = [C_A/BEC50_A] + [C_B/BEC50_B], in which BEC50_A and BEC50_B are the BEC50 values (biofilm eradication concentration resulting in 50% remaining biofilm metabolic activity) of compound A and B alone, while C_A and C_B are the concentrations of the drugs A and B at iso-effective combinations, respectively. Note that C_B values were derived from the whole dose-response curves, whereas C_A values represent the actual concentration of compound A in the checkerboard experiments.