Figure 3. Lgl functions in the elongation of microridges in the apical domain.

Immuno-localization of Lgl and F-actin in the apical domain of peridermal cells in given genetic conditions or morpholino injections at 48 hpf (a). Visualization of the distribution of ridge lengths and medians at 48 hpf, in various genetic conditions mentioned, using bean plots and horizontal lines, respectively (b) followed by estimation of percentage frequency distribution of ridges in short (0–5 μm), intermediate (5–20 μm), long (20–100 μm) and very long (>100 μm) categories (c). Estimation of mean and variance in ridge length for individual cells and comparison of the distributions of the means and variances across various genotypes using box-whisker plots (d). In b and d the distributions denoted by same alphabet or distributions that share an alphabet in common (when denoted by multiple alphabets) do not differ significantly (Dunn's multiple comparisons test, P-value<0.05; see Methods section for details). In c s.d. is shown by error bars. Scale bar in a is equivalent to 10 μm. MO, morphant/morpholino; Std, standard.