Table 2.

Results of testing equine fecal samples experimentally inoculated with different Salmonella enterica isolates using commercially available rapid tests and aerobic culture

Serogroup	Serotype	Total Isolates	Positive Test Results
			LFI‐Aa	LFI‐Bb	DNAHc	qPCRd	TET Culture 1e	TET Culture 2e	BPW Culturef
B	Typhimurium	10	10	10	9	10	10	10	10
	Typhimurium var. 5‐	12	12	11	12	12	12	12	12
C1	Mbandaka	9	8	0	9	8	9	9	9
	Montevideo	15	13	0	12	15	15	15	15
C2	Muenchen	5	5	5	5	5	5	5	5
	Newport	10	9	10	10	10	10	10	10
C3	Kentucky	9	7	9	7	9	9	9	9
E1	Meleagridis	15	14	14	15	15	15	15	15
	Muenster	13	12	13	13	13	13	13	13
K	Cerro	14	4	3	13	14	14	14	14
Total		112	94	75	105	111	112	112	112

Inoculating dose and culture method: ~100 cfu in 1 g of feces with 9 mL of TET broth, incubated for 18 hours at 43°C.

BPW, buffered peptone water; DNAH, DNA hybridization; TET, tetrathionate.

^aReveal® for Salmonella Test System, Neogen® Corporation, Lansing, MI.

^bRapidChek® SELECT^™ Salmonella Test System, SDIX, Newark, NE.

^cGeneQuence® for Salmonella, Neogen® Corporation, Lansing, MI.

^dMicroSEQ® Salmonella spp. Detection Kit, Applied Biosystems^™, Carlsbad, CA.

^eCulture performed on different aliquots of same TET enrichment, plating on XLT‐4 agar that was incubated for 24 hours at 43°C.

^fAliquots of TET enrichment were incubated in BPW for 6 hours at 43°C, then plated on XLT‐4 agar and incubated for 24 hours at 43°C.