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. 2016 Jun 1;7:11432. doi: 10.1038/ncomms11432

Figure 10. SIKE regulates TBK1 through direct physical interaction.

Figure 10

(a) Representative confocal images demonstrate the co-localization of TBK1 and SIKE in the cytoplasm of HEK293T cells. (b,c) HEK293T cells were co-transfected with indicated plasmids. After 48 h, cells were harvested, and cellular lysates were subjected to IP with antibodies against Flag (b) or Myc (c). (d) Immunoblot of a GST pull-down assay, in which exogenous GST-SIKE fusion protein purified from E. coli was incubated with immunopurified Flag-TBK1 from HEK293T cells. (e,f) Immunoblotting with a SIKE or TBK1 antibody was performed on co-IP of Tbk1 from cardiac cell lysates using a TBK1 antibody (e) or SIKE antibody (f). (g,h) Schematic of the full-length and truncated mutants of SIKE (g) and TBK1 (h). (i,j) Immunoblotting was performed with indicated antibodies, following co-IP of the full-length and truncated mutants of SIKE (i) or TBK1 (j) from HEK293T whole-cell lysates using a Myc or Flag antibody, respectively.