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. 2016 Jun 1;7:11432. doi: 10.1038/ncomms11432

Figure 12. Sike-null rats exacerbate pressure overload-induced cardiac hypertrophy.

Figure 12

(a) Immunoblot and quantification of Sike, Anp and β-Mhc protein levels in the hearts of s.d. rats 4 or 8 weeks after sham or AB surgery, *P<0.05 versus the sham-operated group. n=4 rats per group. (b–d) Comparison of the HW/BW (b), LW/BW (c) and HW/TL (d) ratios in different genotypic rats (Sike+/+ and Sike−/−) subjected to sham or AB surgery, n=10–12 rats per group. (e) Histological analyses of heart sections stained with H&E (the first row; scale bar, 50 μm), WGA (the second row; scale bar, 20 μm) or PSR (the third and fourth row; scale bars, 50 μm) in the indicated groups 4 weeks after sham or AB surgery, n=6 or 7 rats per group. (f) Comparison of the cross-sectional area of cardiomyocytes in the indicated groups, n≥100 cells per group. (g) Comparison of the LV collagen volume in the indicated groups, n≥40 fields per group. (h) Immunoblotting and quantification of the active levels of Akt signalling components, n=4 independent experiments. *P<0.05 versus the sham-operated Sike+/+ rat group; #P<0.05 versus the AB-operated Sike+/+ rat group. Data are presented as the mean±s.d. from at least three independent experiments. Statistical analysis was carried out by one-way analysis of variance.