Table 3. The Frequency of the Lactobacillus Species, Identified by Sequencing the DGGE bands Amplified from the Fecal DNA of the RD and H Groups, Using the Lactobacillus and Bifidobacterium Group Specific Primers a,b,c.
| Amplicon ID | Closest relative (Accession number) | Size, bp | H = 15, No. (%) | RD = 15, No. (%) |
|---|---|---|---|---|
| L1, L2, L3, L4 | L. brevis (jx003598) | 1360 | 13 (87) | 13 (87) |
| L5, L6, L7 | L. helveticus (fj749441) | 1462 | 15 (100) | 3 (20) |
| L8, L9, L10 | L. acidophilus (ay763430) | 1475 | 15 (100) | 2 (13) |
| L11, L12, L13, L4 | L. crispatus (jq805668) | 1503 | 14 (93) | 14 (93) |
| L15, L16 | L. fermentum (gq455406) | 1523 | 12 (80) | 0 (0) |
| B1, B2, B3, B4, B5 | B. breve (gu942826) | 294 | 12 (80) | 12 (80) |
| B6, B7, B8, B9, B10 | B. catenulatum (ab846175) | 591 | 15 (100) | 15 (100) |
| B11, B12, B13 | B. infantis (bif16srrge) | 642 | 14 (93) | 0 (0) |
| B15, B17, B18 | B. longum (hq591348) | 1369 | 11 (73) | 0 (0) |
| B16, B21 | B. breve (ay172656) | 1401 | 6 (40) | 0 (0) |
| B14, B19, B20, B22 | B. adolescentis (hq259739) | 1460 | 12 (80) | 5 (33) |
| B23, B24, B25 | B. bifidum (aj311604) | 1469 | 14 (93) | 0 (0) |
aThe marks corresponding to the DGGE bands shown in Figures 3, and 4.
bThe sequence identity was ≥ 99%.
cSignificant differences between RD infants and H infants: P < 0.05.