Skip to main content
. 2016 Jun 3;7:11685. doi: 10.1038/ncomms11685

Figure 5. PsAvh262 and GmBiP1 co-localize around haustoria during infection.

Figure 5

In all panels, proteins were expressed in N. benthamiana through agroinfiltration. The leaves were inoculated with P. infestans zoospores 2 days after agroinfiltration. Images were taken 2 days after inoculation with the relevant P. infestans strain. (a) GFP-SP-GmBiP1-HDEL accumulates around haustoria during infection by P. infestans strain 88069td expressing cytoplasmic tdTomato. Scale bars, 15 μm. (b) GFP-PsAvh262 and SP-GmBiP1-HDEL-RFP co-localized around haustoria following inoculation with untagged P. infestans strain T30-4. White arrows indicate the tips of haustoria. a.u., arbitrary units. Scale bars, 10 μm. (c) GFP-PsAvh262 and RFP-Avrblb2 (RFP attached to the N terminus of AvrBlb2 without its signal peptide) were co-localized around haustoria during infection by untagged P. infestans strain T30-4. In b and c, the fluorescence plots show the relative fluorescence along the dotted lines in the images. Arrowheads point to the tips of haustoria. a.u., arbitrary units. Scale bar, 10 μm.