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. 2016 Mar 18;310(10):H1349–H1359. doi: 10.1152/ajpheart.00939.2015

Fig. 7.

Fig. 7.

Phosphorylated Akt (pAkt, active form) was decreased and proapoptotic Bcl-2-associated death promoter (BAD) protein level was significantly elevated in the PO heart. LCV partially restored pAkt and BAD protein levels to control values. A: representative Western blots probed for pAkt and pBAD proteins in control (Con), PO + LCV, PO + RCV, and PO + sham (n = 5) heart extracts. Total BAD protein levels are also shown. Ponceau S (Pon.s) staining is shown as a protein-loading control. B and C: densitometry analysis of pBAD and total BAD protein band intensity for all Western blots. *P < 0.05, PO + sham vs. Con, PO + LCV, and PO + RCV (by ANOVA followed by Newman-Keuls post hoc analysis). D: ratio of pBAD to BAD. No significant difference was observed. E: densitometry analysis of pAkt protein level (n = 4). *P < 0.05, Con vs. all PO; #P < 0.05, PO + LCV vs. PO + RCV and PO + sham.