Skip to main content
. 2016 Mar 11;310(10):G844–G854. doi: 10.1152/ajpgi.00407.2015

Fig. 5.

Fig. 5.

In colonic cells, TNF-stimulated intracellular IL-8 protein is associated with the surface of LDs in endoplasmic reticulum (ER) and Golgi. A: HT-29 cells treated with TNF (6 h) with and without C75 were immunofluorescently stained with IL-8 antibody followed by secondary antibody (Alexa 594) and BODIPY 493/503, or IL-8 antibody followed by secondary antibody, or BODIPY 493/503 only, or IgG-only secondary antibody (Alexa 594) (representative images from 3 independent experiments, scale bar = 5 μm). B: graphical representation of IL-8 distribution between LD-positive and LD-negative cells (n = 300 cells). C: orthogonal view of confocal images of PLIN2 (Alexa 488) and IL-8 (Alexa 594). White arrows show colocalization of LDs and IL-8 (blue: nucleus, green: PLIN2, red: IL-8, scale bar = 3 μm). D: orthogonal view of confocal images of BODIPY 493/503, IL-8 (Alexa 594), and calnexin (Alexa 647). White and yellow arrows show colocalization of 2 and 3 colors, respectively (gray: nucleus, green: LDs, red: IL-8, blue: calnexin, scale bar = 3 μm). E: orthogonal view of confocal images of BODIPY 493/503, IL-8 (Alexa 594), and syntaxin 6 (Alexa 647). White and yellow arrows show colocalization of 2 and 3 colors, respectively (gray: nucleus, green: LDs, red: IL-8, blue: syntaxin 6, scale bar = 3 μm).