Figure 2.

Expression kinetics of cytokines mRNA. A–F Relative expression of cytokines mRNA, accounting for the effects of culture conditions on gene transcription in WNV- and mock-inoculated equine PBMCs. To compare the normalised expression of cytokine genes from PBMCs harvested at each time point to their respective expression levels before either WNV- or mock- inoculation, the ∆∆Ct values were calculated by subtracting ∆Ct of genes in fresh-isolated PBMCs from the ∆Ct of genes in WNV- or mock- inoculated PBMCs at each time-point (for WNV-stimulated PBMCs, $\mathrm{\Delta }\mathrm{\Delta }{\text{Ct}}_{\text{WNV}}=\mathrm{\Delta }{\text{Ct}}_{\text{WNV}}-\mathrm{\Delta }{\text{Ct}}_{\text{fresh}}$; and for mock-inoculated PBMCs, $\mathrm{\Delta }\mathrm{\Delta }{\text{Ct}}_{\text{mock}}=\mathrm{\Delta }{\text{Ct}}_{\text{mock}}-\mathrm{\Delta }{\text{Ct}}_{\text{fresh}}$). *P < 0.05.