Fig. 3.
Simulation comparison of four simplified rate laws against a reference module containing detailed enzyme mechanism kinetics (enzyme modules). The responses of metabolites under different perturbations were compared between four simplified rate laws and the enzyme module. a Correlation of metabolite relaxation time. b Correlation of metabolite maximum perturbation. c Median percent errors of metabolite relaxation time. d Median percent errors of metabolite maximum perturbation. Nine different perturbations labeled from 1 to 9 were performed. 1, ATP, ADP and Pi perturbation; 2, NAD and NADH perturbation; 3, 23DPG perturbation; 4, 3PG perturbation; 5, PYR perturbation; 6, FDP perturbation; 7, PRPP perturbation; 8, MAN6P perturbation; 9, R5P perturbation. Spearman’s rho: Spearman’s rank correlation coefficient. The simulations were performed on the whole-cell kinetic model of erythrocyte constructed by Bordbar et al [33]