Table 1.
General description of the constructed enzyme modules
| Enzyme name | Module size (metabolites × reactions) | Regulators (mechanism of action) |
|---|---|---|
| Phosphogluconate dehydrogenase (GND) | 13 × 9 | NADPH (PI) |
| Lactate dehydrogenase (LDH) | 10 × 6 | N/A |
| Glucose-6-phosphate dehydrogenase (G6PDH) | 12 × 7 | ATP (CI), NADPH (PI) |
| Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | 27 × 27 | 3PG (AI), G3P (AI) |
| Hexokinase (HEX1) | 10 × 6 | 23DPG (CI) |
| Pyruvate kinase (PK) | 30 × 34 | FDP (AA), ATP (PI, AI) |
| Phosphofructokinase (PFK) | 40 × 44 | ADP (PI), ATP (AI), AMP (AA) |
| Phosphoglycerate kinase (PGK) | 13 × 9 | ATP (PI), 3PG (PI), 23DPG (CI) |
| Adenylate kinase (ADK) | 8 × 5 | N/A |
| Glucose-6-phosphate isomerase (PGI) | 5 × 3 | N/A |
PI product inhibitor, AI allosteric inhibitor, AA allosteric activator, CI competitive inhibitor