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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Cancer Res. 2016 Mar 28;76(11):3295–3306. doi: 10.1158/0008-5472.CAN-15-2197

Figure 3. Tamoxifen treatment promotes glycolysis and alters fatty acid metabolism.

Figure 3

A. Glucose uptake from media following tamoxifen treatment for 1 hour in MCF7 and MDA-MB-231 cells. Data are the average of three independent experiments ±SEM *P<0.05 **P<0.01 ***P<0.001. LC-MS/MS analysis of 13C6-glucose metabolites after 30μM tamoxifen treatment for 1 hour in MDA-MB-231 cells: Mass isotopomer analysis of B. Acetyl CoA and C. Lactate. N=4, **P<0.01 ***P<0.001. D. Immunoblot analysis of phos-AMPK and total AMPK of cells treated with 20μM tamoxifen over time under glucose deprivation or 5mM glucose. E. Immunoblot analysis of LC3-I and LC3-II in BC cells after tamoxifen treatment.