Skip to main content
. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Cancer Res. 2016 Mar 28;76(11):3295–3306. doi: 10.1158/0008-5472.CAN-15-2197

Figure 4. AMPKα1 and AMPKα2 mediate divergent effects on downstream effectors and cellular survival after tamoxifen treatment.

Figure 4

A. Immunoblot blot of AMPK pathway activation and apoptotic markers after tamoxifen treatment. Values below blots represent pixel intensity normalized to loading control, β-Actin. B. Immunoblot of LC3-I and LC3-II flux with tamoxifen treatment as affected by AMPK status. Values represent Pixel intensity of LC3-I (top) or LC3-II (bottom) normalized to untreated control. C. Survival of AMPK knock down cells treated with 30μM tamoxifen for 4 hrs. Data are the average of four independent experiments ±SEM *P<0.05 **P<0.01 ***P<0.001C. D. Quantification of 35S labeling after tamoxifen treatment in MDA231 cells and AMPK CRISPR knock out cells. Data are the average of three independent experiments ±SEM *P<0.05.