Fig. 4.

Overexpression of mouse Iah1 in Hepa1-6 cells. a The Iah1 mRNA level in non-transfected (control) and mouse Iah1 cDNA-transfected (Iah1) cells. Iah1 cells were shown to stably overexpress mouse Iah1 protein. b The Iah1 protein level in control cells and mouse Iah1 cells. c The mRNA levels of genes related to lipid metabolism (n = 4–5; *P < 0.05, **P < 0.01 versus A/J mice). d The cellular TG contents in non-transfected (control) and mouse Iah1 cDNA-transfected (Iah1) cells. The treatment medium consisted of serum-free medium plus 0.333 mM oleic acid and was administered to the treatment group for 48 h. The basal group was cultured in serum-free medium without oleic acid. The analyses were performed in duplicate, and at least two independent experiments were performed. The data were analyzed by two-way ANOVA. Oleic acid effect, P < 0.05; Iah1 effect, not significant; Oleic acid × Iah1 (interaction) effect, not significant