Fig. 5.
Role of IFN-γ in the control of dM infection by dNK cells. a CD119 expression was analysed on dMs by flow cytometry. Values depicted on the graph are the percentages of expression of CD119 among dMs from 5 different donors. The median is also displayed. A representative FACS histogram gated on CD45+ CD14+ cells is shown (filled grey profile, anti-CD119 antibody and white profile, control). b Representation of the experimental system. Double chamber coculture supernatant (SN) from one donor (donor no. 1) were treated or not with an IgG isotype control or an anti-IFN-γ antibody. dMs from another donor (donor no. 2) infected with HIV-1BaL at an MOI of 10−3 one hour before, were then incubated during 3 days with these supernatants. Viral production was followed by the quantification of the p24 Ag in the supernatants. Each supernatant was incubated with dMs from a different donor. c The percentage of inhibition of the infection was calculated at the time point corresponding to the largest dM viral production inhibition. For each donor, all conditions were performed. Each symbol represents one donor. The medians of the percentage of inhibition from 4 donors are displayed. d and e dMs were infected with HIV-1BaL at an MOI of 10−3, treated with recombinant human IFN-γ (5 or 100 ng/mL) during 3 days and then left in culture in an IFN-γ free medium. Viral production was followed as previously described. d The viral production kinetics for a representative donor is displayed over time. e The percentage of inhibition of the infection of dMs treated with 5 ng/ml of IFN-γ was calculated at the time point corresponding to the largest dM viral production inhibition. The median of the percentage of inhibition from 3 donors is displayed