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. 2016 Jun 6;213(5):557–570. doi: 10.1083/jcb.201504043

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© 2016 Tatomer et al.

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Figure 6. — Histone mRNA expression analyzed by in situ hybridization. (A) RNA pol II occupancy data for 14–16 h embryos and Kc167 cells (Kharchenko et al., 2011) visualized on a single histone repeat. The location of in situ hybridization probes used in B is indicated. (B) Fluorescent in situ hybridization of ovaries of the indicated genotypes using an H3 coding probe or a probe (H3-ds) that only detects misprocessed or read-through transcripts. Note that histone H3 mRNA accumulates only during S phase and that the nurse cell endocycles are asynchronous. The cytoplasmic H3-ds signal in FLASH^PBac/Df is from misprocessed H3 transcripts that were polyadenylated and exported. The small amounts of misprocessed RNA in FLASH¹⁻⁷³³ and mxc^G46 (see Fig. 5 B) mRNAs are below our threshold of detection. The nuclear foci in FLASH^1-733 and mxc^G46 mutants detected with the H3-ds probe (yellow arrows) are nascent transcripts at the histone locus. Bars, 10 µm.