Table 3. Construct features.
| FLASH transgene feature | Sequence |
|---|---|
| attB–KpnI–promoter | 5′-GATCTCTAGAGGTACCcgagattattgtttgtattgtgatgtttttttttaacgaatttaa-3′ |
| V5–XhoI–AttB | 5′-GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAACTCGAGCCGCGGCCGCAGAT-3′ |
| 5′ UTR–EcoRI–coding | 5′-GAAGAAACGTAAGCGATTGGTGAATTCCAAAATG-3′ |
| EcoRI–FL | 5′-GAATTCCAAAATGGAAACGCCTGCATATGCCAC-3′ |
| EcoRI–aa 65 | 5′-GAATTCCAAAATGGACAGATCCCTTGAACTGGAC-3′ |
| EcoRI–aa 78 | 5′-GAATTCCAAAATGGACGACTTTCAGAAGGCCGA-3′ |
| aa 844–SacII–V5 | 5′-CTGCTGCgGACAAACCAATTCCGCGGTTCGAA-3′ GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG-3′ |
| aa 733–SacII–V5 | 5′-CTCACCCAGACTCCAAAACAGGCTCCGCGGTTCGGA-3′ GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG-3′ |
| FLASHmini junction | 5′-CGAAAAGGACGATGT/ACCCAGACTCCAAAA-3′ |
Engineered restriction sites are underlined, start and stop codons are bold, the FLASH promoter sequence is lowercase, the V5 tag is italicized, and “/” indicates the unique junction in the FLASHmini open reading frame.