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| Tissue | Fixation | Permeablization |
|---|---|---|
| Salivary gland (except in Figs. 4 C and 5 A) | 7% formaldehyde, 20 min | 0.2% Tween-80, 15 min |
| Salivary gland (Fig. 4 C) | 3.7% formaldehyde, 20 min | Not performed |
| Salivary gland (Fig. 5 A) | 3.7% formaldehyde, 15 min | 1% Triton X-100, 15 min |
| Ovary | 7% formaldehyde, 20 min | 0.2% Tween-80, 15 min |
