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. 2016 Jun 6;213(5):525–534. doi: 10.1083/jcb.201602007

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© 2016 Aaltonen et al.

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Figure 2. — PE biogenesis can proceed in the absence of Ups2. (A) PE/PC biogenesis in S. cerevisiae. Thick arrows represent the reactions proceeding in the experiments in B and in Fig. 4 A, where nonmitochondrial PE/PC synthesis was shut down by deletion of DPL1 and PSD2 and the lack of ethanolamine (Etn) or choline (Cho) in the medium. Red arrows represent lipid exchange. (B) Assessment of PE/PC synthesis in vivo. After pulse labeling with [¹⁴C]serine and further incubation in medium with unlabeled serine (chase), lipids were extracted and analyzed by TLC and autoradiography. ΔΔ, dpl1Δpsd2Δ. (C) Levels of PS, PE, and PC were quantified and are shown as a fraction of total lipids. Error bars represent SD. n = 3.