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. 2016 Jun 6;213(5):525–534. doi: 10.1083/jcb.201602007

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© 2016 Aaltonen et al.

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Figure 3. — Psd1 is able to convert PS to PE at juxtaposed membranes in trans. (A) Monitoring of trans-decarboxylation by reconstituted Psd1. (B) Autocatalytic processing of reconstituted Psd1. The Psd1-containing fraction after flotation was subjected to SDS-PAGE and immunoblot analysis using HA-specific antibodies. CBB, Coomassie brilliant blue. (C) Psd1 decarboxylates PS to PE at juxtaposed membrane. S463A, Psd1^S463A. Spontaneous PS transfer under identical conditions was plotted for comparison. PE produced or PS transferred at given time points are shown as percentage of total PS input. Error bars represent SEM. n = 3.