Table 6.
Comparison of custom and FTD assay in regard to sample testing
| Custom assay | FTD assay | |
|---|---|---|
| Viral Pathogens | Flu A, Influenza | Flu A, Influenza |
| A(H1N1)pdm09, Flu B | A(H1N1)pdm09, Flu B | |
| HPIV - 1, 2, 3, 4 | HPIV - 1, 2, 3, 4 | |
| HCoV OC43, 229E, NL63, | HCoV OC43, 229E, NL63, | |
| HKU1, | HKU1, | |
| RSV A/B | RSV A/B | |
| HMPV A/B | HMPV A/B | |
| EV | EV | |
| HPeV | HPeV | |
| HRV | HRV | |
| HAdV | HAdV | |
| HBoV | HBoV | |
| -- | M. pneu | |
| Sample volume | 200 μl | 200 μl |
| Elution volume | 55 μl | 55 μl |
| PCR volume | 25 μl | 25 μl |
| Principle of detection | Fluroscence probe based | Fluroscence probe based |
| Hands on time | 50 min | 50 min |
| Time to result | 3.5 h | 3.01 h |
| Total cost per sample | INR 1500 | INR 4300 |
| Ease of Handling | + | + |
| Equipment | NucliSENS EasyMAG, ABI 7500 DX Fast | NucliSENS EasyMAG, ABI 7500 DX Fast |
| No. of PCR tubes used per sample | 6 | 5 |
| Primers & probes | Custom assay (ABI) | FTD |
| Number of targets/tube | 3 | 4 |
| PCR reaction volumes | ||
| 2X RT-PCR buffer | 12.5 μl | 12.5 μl |
| 25X RT-PCR enzyme | 1.0 μl | 1.0 μl |
| Primer probe mix/tube | 1.8 μl | 1.5 μl |
| Nucleic acid | 9 μl | 10 μl |
| Nuclease free water | 0.7 μl | -- |