Fig. 4.
Changes in α-SMA expression and other genes associated with BPD were specific to FLFs cultured in 3D. A: IF for α-SMA and vimentin expression and BPD target gene expression pattern by qPCR in 2D FLF cultured in normoxia and hypoxia-hyperoxia conditions. Scale bar = 50 μm. B: IF for α-SMA and vimentin expression and BPD target gene expression pattern by qPCR in 3D skin fibroblasts cultured in normoxia and hypoxia-hyperoxia conditions. Scale bar = 50 μm. C: IF for α-SMA and vimentin expression and BPD target gene expression pattern by qPCR in 3D adult primary fibroblasts cultured in normoxia and hypoxia-hyperoxia conditions. Scale bar = 50 μm. Triplicate samples were used in each experiment. Experiments were repeated a minimum of 3 times. *P < 0.001.