Skip to main content
. 2016 Mar 18;310(10):L993–L1002. doi: 10.1152/ajplung.00046.2016

Fig. 2.

Fig. 2.

Expression of recombinant MUC5B COOH-terminal protein domain. A: schematic showing the domains of the COOH-terminal construct [CT5B; D4-B-C-CK domains (open ovals), His-Tag (solid square)] of MUC5B. B: the expressed COOH-terminal construct was analyzed by SDS-PAGE [reduced (R) and nonreduced (NR)] and stained with InstantBlue. Different CT5B protein preparations yielded varying proportions of monomer and dimer. C: reduced CT5B was incubated with (+) or without (−) PNGase F and analyzed by SDS-PAGE and stained with InstantBlue. D: SEC-MALLS analysis of CT5B showing light scattering (dashed line) and refractive index (solid line); molecular mass values of CT5B monomer and dimer are shown.