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. Author manuscript; available in PMC: 2016 Jun 7.
Published in final edited form as: Spine J. 2011 Oct 22;12(1):7–20. doi: 10.1016/j.spinee.2011.09.011

Fig. 2.

Fig. 2

Construction of recombinant adeno-associated virus (AAV) vectors. Schematic illustration of two AAV vectors containing the human bone morphogenetic protein 2 (hBMP2) gene and human tissue inhibitor of metalloproteinase 1 (hTIMP1) gene were constructed as described. Both vectors were controlled by the human cytomegalovirus (CMV) immediate-early promoter/enhancer and followed by the simian virus 40 polyadenylation signal (SV40_pA). The difference between two vectors is that the BMP2 gene (1,191 bp) was cloned into a single-stranded AAV vector [51], and the complementary DNA of TIMP1 (624 bp) was cloned into a self-complementary AAV vector [52]. ITR, inverted terminal repeat; Pcmv, human cytomegalovirus immediate-early promoter/enhancer; SD/SA, the SV40 late viral protein gene 16s/19s splice donor and acceptor signals; POenh, a tandem repeat of the enhancer from the polymavirus mutant PYF441; Ptk, the thymidine kinase promoter of herpes simplex virus; neor, the neomycin gene from Tn5; Bgh_pA, the bovine growth hormone polyadenylation signal from pRc/CMV (Invitrogen); 6ITR, mutant ITR.