Figure 1.

Illustration of our finalized WTTS-seq library preparation procedures. Total RNA serves as the starting material, followed by fragmentation and poly(A)+ RNA enrichment. Reverse transcription synthesizes the first-strand cDNA and adds both 5′- and 3′-adaptors into the library. Treatment with RNases I and H removes all RNA molecules and leaves the first-strand cDNA alone for second-strand synthesis by PCR. The library is then size selected and ready for NGS.