Figure 10.

Expression patterns of overlapping genes are well detected by WTTS-seq but not by RNA-seq. Partial genome regions of X. tropicalis lamb1 and dld genes overlap in opposite directions. The WTTS-seq libraries produced at least two major clusters of reads also with opposite directions in the overlapping region. The blue reads were derived from lamb1 and the red reads from dld. Reads in the RNA-seq libraries covered the overlapping region, but there was no way to allocate them to each gene. Furthermore, RNA-seq mapping quality in the overlapping region was quite low (see reads pointed out with arrows).