Figure 8.

APA patterns during embryo development are revealed by WTTS-seq but not by RNA-seq. Partial genomic region of X. tropicalis ubtd1 gene including the last two exons is shown. WTTS-seq revealed that the distal APA site was dominant at stage 6, but usage switched to the proximal site at stage 11. At stage 28, however, both sites were used equally. Unfortunately, RNA-seq failed to reveal any differences in usage of proximal or distal APA sites among these five stages. The poly(A) site signals were presented proportionally for each family at each stage but disproportionally among different stages.