Table 2 .

Expected prediction error under various experimental designs

Experimental design	Type of mutation*	Landscapes using this protocol	n			Wmax			σ			Q
			rej	reg	nn	rej	reg	nn	rej	reg	nn	rej	reg	nn
5 mutations, 25 genotypes	R	A, C	0.85	0.68	0.64	0.57	0.39	0.37	0.44	0.35	0.32	0.67	0.49	0.43
	IS	-	0.91	0.79	0.7	0.34	0.2	0.18	0.33	0.19	0.19	0.35	0.15	0.09
	CS	F	0.83	0.73	0.63	0.34	0.19	0.17	0.53	0.37	0.33	0.39	0.24	0.17
4 mutations, 24 genotypes	R	-	0.93	0.8	0.78	0.79	0.58	0.54	0.42	0.36	0.35	0.64	0.52	0.45
	IS	I	0.87	0.76	0.67	0.41	0.22	0.18	0.43	0.25	0.23	0.48	0.27	0.2
	CS	G	0.9	0.76	0.69	0.37	0.2	0.17	0.49	0.33	0.3	0.42	0.33	0.24
8 mutations, 8 single and 20 double mutants	RS	-	0.8	0.58	0.54	0.68	0.48	0.5	0.37	0.3	0.29	0.55	0.44	0.4
	IS	-	0.75	0.69	0.63	0.44	0.29	0.22	0.41	0.29	0.28	0.47	0.33	0.29
	CS	-	0.77	0.67	0.62	0.4	0.21	0.18	0.43	0.26	0.23	0.27	0.23	0.2
20 mutations, up to 121 genotypes	R	B	0.72	0.62	0.54	0.48	0.25	0.21	0.35	0.23	0.22	0.62	0.45	0.39
9 mutations, 9 single mutants, 18 double mutants	IS	D	0.74	0.68	0.63	0.37	0.22	0.15	0.38	0.27	0.24	0.45	0.37	0.32
6 mutations, 6 single mutants, 15 double mutants	IS	E	0.81	0.8	0.76	0.45	0.25	0.18	0.39	0.33	0.3	0.67	0.59	0.54
5 mutations, 25 genotypes	IS, high fitness combination	H1, H2	0.86	0.74	0.61	0.24	0.09	0.06	0.42	0.25	0.22	0.24	0.1	0.05
4 mutations, 24 genotypes	IS, small fitness effect mutants	H3	0.8	0.84	0.78	0.69	0.52	0.43	0.5	0.41	0.38	0.69	0.48	0.36
4 mutations, 24 genotypes	IS, large fitness effect mutants	H4	0.94	0.72	0.56	0.26	0.1	0.07	0.25	0.15	0.14	0.27	0.1	0.04

Prediction error for the four parameters of Fisher’s model, for several experimental designs (based on single and double mutants, or complete sets of mutations and all associated genotypes) and selection procedures (* R: random, IS: independently selected, CS: co-selected mutations), when the 6 summary statistics were used in the ABC algorithm. For each parameter, the three lowest prediction errors are in bold, highlighting the protocol and inference algorithms that perform best.