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. 2016 Jun 7;7:789. doi: 10.3389/fpls.2016.00789

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Copyright © 2016 Deng, Chen, Leclercq, Zhou, Liu, Liu, Yang, Montoro, Xia and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of the interaction protein of HbTCTP by yeast two-hybird system. (A) Yeast cells transformed with bait and prey vectors were streaked onto SD/-Leu/-Trp/-His. pAD-WT/pBD-WT and pAD-WT/pLamin C were used as positive and negative controls, respectively. (B) β-Galactosidase assays. The numbers 1–11 indicate yeast cells transformed with the following plasmid combinations: 1, pAD-WT/pBD-WT; 2, pAD-MUT/pBD-MUT; 3, pGAL4; 4, pAD-WT/pLamin C; 5, pAD-MUT/pLamin C; 6, pLamin C; 7, pAD-1/pBD-HbTCTP; 8, pAD-2/pBD-HbTCTP; 9, pAD-3/pBD-HbTCTP; 10, pAD-4/pBD-HbTCTP; 11, pAD-5/pBD-HbTCTP. Arrow indicated that the positive clones.