Figure 6.

Protection of supercoiled DNA cleavage by recombinant HbTCTP in a MCO system. (1) pUC19 without incubation; (2) pUC19 in water and incubated 37°C for 2 h; (3) pUC19 only with FeCl₃; (4) pUC19 only with DTT; (5) pUC19 with MCO system; (6) pUC19 with BSA (0.6 mg/mL) with MCO system as a negative control; (7) pUC19 with HbTCTP protein (200 μg/mL) with MCO system; (8) pUC19 with HbTCTP1 protein (200 μg/mL) with MCO system. NF, nicked form; SF, supercoiled form of the plasmid.